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Herpes Çalışmaları A Primer Nöron Kültür Sistem Virüs gecikmesi ve Yeniden Simplex
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1Department of Microbiology,New York University School of Medicine, 2Molecular Neurobiology Program, Skirball Institute for Biomolecular Medicine,New York University School of Medicine, 3Department of Otolaryngology,New York University School of Medicine, 4Department of Cell Biology,New York University School of Medicine, 5Department of Physiology and Neuroscience,New York University School of Medicine, 6Department of Psychiatry,New York University School of Medicine, 7Center for Neural Science,New York University School of Medicine
Chapters
- 00:05Title
- 01:52Isolation and Culture of SCG Neurons from Rat Embryos
- 05:56Infection of SCG Cultures with HSV-1 Us11-EGFP and Establishment of Latency
- 08:35Reactivation and Assessment
- 09:45Results: HSV-1 Reactivation
- 11:19Conclusion
Protokol herpes virüs tip 1 (HSV-1) gecikme ve reaktivasyonu simpleks incelemek için etkin ve tekrarlanabilir model sistemi anlatılmaktadır. Assay homojen sempatik sinir kültürler kullanmaktadır ve RNA etkileşimi ve rekombinant proteinlerin ifadesi de dahil olmak üzere çeşitli araçlar kullanılarak virüs nöron etkileşimlerin moleküler Diseksiyon için olanak sağlar.
