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视网膜神经细胞发育和成熟的单细胞的剖析
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Single-cell Profiling of Developing and Mature Retinal Neurons
DOI:

10:20 min

April 19, 2012

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Chapters

  • 00:05Title
  • 02:07Cell Dissociation
  • 03:44Harvesting Single Cells
  • 05:15Reverse Transcription
  • 06:45Tailing and Single-cell PCR
  • 07:15Labeling for Affymetrix Chips
  • 08:23Results: Single-cell Profiling of Developing and Mature Retinal Neurons
  • 09:42Conclusion

Summary

Automatic Translation

描述一个孤立单一的视网膜细胞,随后他们的cDNA扩增的方法。单细胞转录揭示了在组织细胞的异质性目前的程度,并揭示了罕见的细胞群的新的标记基因。所附的协议,可以调整,以适应多种不同的细胞类型。

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