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Neuroscience
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监测变化的细胞内钙离子浓度和突触效能的软体动物<em>海兔</em
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Monitoring Changes in the Intracellular Calcium Concentration and Synaptic Efficacy in the Mollusc
Aplysia
Please note that all translations are automatically generated.
Click here for the English version.
监测变化的细胞内钙离子浓度和突触效能的软体动物<em>海兔</em
DOI:
10.3791/3907-v
•
09:51 min
•
July 15, 2012
•
Bjoern Ch. Ludwar
,
Colin G. Evans
2
,
Elizabeth C. Cropper
1
Fishberg Department of Neuroscience and Friedman Brain Institute
,
Mt. Sinai School of Medicine
,
2
Phase Five Communications Inc.
Chapters
00:05
Title
01:53
Dissection
03:21
Preparing electrodes
04:40
Loading the calcium indicator dye
05:27
Calcium imaging and electrophysiological recording
07:57
Analysis
08:40
Representative data
09:18
Conclusions
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们演示了如何在细胞内游离钙离子浓度和突触效能的变化,可以同时监测神经节准备的<em>海兔</em>。我们的图像使用的荧光染料,钙奥兰治,用锋利的(细胞内)电极和诱导和监测突触传递的细胞内钙离子。
Tags
Intracellular Calcium
Synaptic Efficacy
Mollusc Aplysia
Calcium Imaging
Intracellular Recording
Buccal Ganglion
Synaptic Plasticity
Homosynaptic Facilitation
Calcium Orange
Iontophoresis
Fluorescent Dye
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