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监测变化的细胞内钙离子浓度和突触效能的软体动物<em>海兔</em
JoVE Journal
Neuroscience
Author Produced
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JoVE Journal Neuroscience
Monitoring Changes in the Intracellular Calcium Concentration and Synaptic Efficacy in the Mollusc Aplysia
DOI:

09:51 min

July 15, 2012

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Chapters

  • 00:05Title
  • 01:53Dissection
  • 03:21Preparing electrodes
  • 04:40Loading the calcium indicator dye
  • 05:27Calcium imaging and electrophysiological recording
  • 07:57Analysis
  • 08:40Representative data
  • 09:18Conclusions

Summary

Automatic Translation

我们演示了如何在细胞内游离钙离子浓度和突触效能的变化,可以同时监测神经节准备的<em>海兔</em>。我们的图像使用的荧光染料,钙奥兰治,用锋利的(细胞内)电极和诱导和监测突触传递的细胞内钙离子。

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