/
/
Microfluidica Endothelialized per lo studio delle interazioni microvascolari nelle patologie ematologiche
A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Department of Pediatrics,Emory University School of Medicine, 2Wallace H. Coulter Department of Biomedical Engineering,Georgia Institute of Technology and Emory University, 3Aflac Cancer Center and Blood Disorders Service of Children’s Healthcare of Atlanta , 4Winship Cancer Institute of Emory University
Chapters
- 00:05Title
- 01:11Fabrication of the Endothelial Microdevice
- 03:46Polydimethylsiloxane (PDMS) Preparation
- 06:25Seeding the Microfluidic Device with Endothelial Cells
- 08:49Endothelialized Microfluidics Reveal Abnormal Microvascular Interactions after Infusion with Blood from Patients with Hematologic Disease
- 10:35Conclusion
Un metodo per la cultura un monostrato di cellule endoteliali durante l'intera superficie interna 3D di un dispositivo a microfluidi con microvascolare canali di dimensioni (<30 micron) è descritta. Questo<em> In vitro</em> Modello microvascolarizzazione consente lo studio di interazioni tra le cellule del sangue biofisiche, cellule endoteliali, e fattori solubili in malattie ematologiche.
Tags
MicrofluidicsEndothelialized MicrofluidicsMicrovascular InteractionsHematologic DiseasesMicrofabrication TechniquesBiological ExperimentsBiochemical ExperimentsMicro- And NanoscalesHematologic ProcessesBlood Cell DeformabilityBlood Cell AggregationMicrovascular Blood FlowBlood Cell-endothelial Cell InteractionsCultured Endothelial CellsMicrocirculationBiophysical Pathophysiology
