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Oct4GiP Reporter Assay til at studere gener, der regulerer mus embryonale stamceller Vedligeholdelse og Self-fornyelse
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JoVE Journal Biology
Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal

Oct4GiP Reporter Assay til at studere gener, der regulerer mus embryonale stamceller Vedligeholdelse og Self-fornyelse

DOI:
10.3791/3987-v

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08:01 min

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May 30, 2012

•

,
1Laboratory of Molecular Carcinogenesis,National Institute of Environmental Health Sciences

Chapters

  • 00:05Title
  • 01:38siRNA Transfection in Oct4GiP Cells
  • 04:05FACS Analysis
  • 06:07Results: The Oct4GiP Reporter Assay Readily Detects the Differentiation Caused by Gene Silencing in the Maintenance of ES Cell Renewal
  • 07:30Conclusion

Summary

Automatic Translation

Automatic Translation

Beskriver vi en fluorescens Reporter Assay til hurtigt at identificere og karakterisere gener, der regulerer muse embryonale stamceller vedligeholdelse og selvfornyelse.

Tags

Oct4GiPReporter AssayGenesRegulateMouse Embryonic Stem Cell MaintenanceSelf-renewalPluripotencyMolecular MechanismDevelopmental Biology StudiesDisease ModelingDrug DiscoveryRegenerative MedicineFluorescence Reporter-based AssaySelf-renewal StatusMouse ES CellsOct4 Gene Promoter RegionGreen Fluorescent Protein (GFP)DifferentiationFluorescence-activated Cell Sorting (FACS) AnalysisRNAiConvenienceSensitivityQuantitative AnalysisCost-effective

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