A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:00Imaging Teratoma Formation
- 01:07Introduction
- 01:47Getting Started: Review of Stem Cell Culture and Imaging Set-Up
- 03:33In Vitro Bioluminescence Imaging of Human Embryonic Stem Cells
- 07:47In vivo Bioluminescence Imaging of Injected hESC’s
- 11:17Conclusion
With the growing interest in stem cell therapies, molecular imaging techniques are ideal for monitoring stem cell behavior after transplantation. Luciferase reporter genes have enabled non-invasive, repetitive assessment of cell survival, location, and proliferation in vivo. This video will demonstrate how to track hESC proliferation in a living mouse.
Tags
In VitroIn VivoBioluminescenceReporter Gene ImagingHuman Embryonic Stem CellsHESCsRegenerative MedicineCell DeathTeratoma FormationHost Immune RejectionTransplantationImaging TechniquesHESC BehaviorLocalizationProliferationViabilityMolecular ImagingSpatio-temporal KineticsEngraftmentTeratoma-formationNoninvasive Reporter Gene AssaysMulti-modality Imaging PlatformsEngineered Promoters And Enhancers
