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1Department of Medicine,Weill Cornell Medical College , 2Department of Oral Biology,University of Missouri-Kansas City-School of Dentistry, 3Department of Pharmacology and Toxicology,University of Missouri Kansas City- School of Pharmacy, 4Regional Hospital,Bamenda, NWP, Cameroon, 5Mezam Polyclinic HIV/AIDS Treatment Center, Cameroon, 6Institute for Human Genetics and Biochemistry
Chapters
- 00:05Title
- 02:31Preparation of Working Solutions
- 03:51Saliva Collection and Handling
- 04:57Microplate Set-up
- 06:55Calculating the alpha1PI Index
- 07:30Representative Assay Results
- 10:11Conclusion
A CD4 enumeration method, the α-test, is described which uses whole saliva to provide rapid and accurate CD4 counts. The α-test costs pennies and eliminates the need for technical training, costly reagents such as monoclonal antibodies, instrumentation, refrigeration, transport of samples, as well as collection and handling of blood.
Tags
Affordable CD4 EnumerationHIV Disease MonitoringResource-limited RegionsTime And Temperature RestrictionsTechnical SophisticationCost Of ReagentsMonoclonal AntibodiesCell-free CorrelatesCD4 Counts CalculationPhysiological RelevanceStem CellsCXCL12CXCR4Cell Surface Human Leukocyte Elastase (HLECS)α1proteinase Inhibitor (α1PI)HIV-1 Disease Processes
