Journal
/
Medicine
/
Characterization of Molecular Mechanisms of In vivo UVR Induced Cataract
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Medicine
Characterization of Molecular Mechanisms of In vivo UVR Induced Cataract

Characterization of Molecular Mechanisms of In vivo UVR Induced Cataract

DOI:
10.3791/4016-v

•

13:56 min

•

November 28, 2012

•

, ,
1St. Erik’s Eye Hospital,Karolinska Institutet, 2Gullstrand lab, Section for Ophthalmology, Department of Neuroscience,Uppsala University

Chapters

  • 00:05Title
  • 02:00Exposure to Ultraviolet Radiation
  • 03:26Dissection
  • 04:31Quantitative RT-PCR
  • 08:56Immunohistochemical Staining
  • 10:06Sectioning and Fluorescence Immunohistochemistry
  • 11:41Representative qPCR and Imaging of UVR Exposed vs Non-exposed Lens
  • 13:30Conclusion

Summary

Automatic Translation

Automatic Translation

Cataract is the leading cause of blindness in the world. Solar ultraviolet radiation (UVR) is the main risk factor for cataract development. An animal model of far UVR-B induced cataract was developed. In this article we describe methods for investigation of cataract formation: exposure to UVR, quantitative RT-PCR and immunohistochemistry.

Tags

CataractBlindnessWorld Health OrganizationClouding Of The LensMulti-factorial DiseaseDiabetesSmokingUltraviolet Radiation (UVR)AlcoholIonizing RadiationSteroidsHypertensionAnimal ModelSurgeryPrevention StrategiesApoptosisLensPharmaceuticalsRT-PCRImmunohistochemistry

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/4016?article_type=v

In vivo Near Infrared Fluorescence (NIRF) Intravascular Molecular Imaging of Inflammatory Plaque, a Multimodal Approach to Imaging of Atherosclerosis
check_url/4016?article_type=v

An in vivo Rodent Model of Contraction-induced Injury and Non-invasive Monitoring of Recovery
check_url/4016?article_type=v

In vivo 19F MRI for Cell Tracking
check_url/4016?article_type=v

Molecular Profiling of the Invasive Tumor Microenvironment in a 3-Dimensional Model of Colorectal Cancer Cells and Ex vivo Fibroblasts
check_url/4016?article_type=v

Chemotherapy-induced Vascular Toxicity – Real-time In vivo Imaging of Vessel Impairment
check_url/4016?article_type=v

Establishment and Characterization of UTI and CAUTI in a Mouse Model
check_url/4016?article_type=v

Depletion of Mouse Cells from Human Tumor Xenografts Significantly Improves Downstream Analysis of Target Cells
check_url/4016?article_type=v

In Vitro and In Vivo Detection of Mitophagy in Human Cells, C. Elegans, and Mice
check_url/4016?article_type=v

A Doxorubicin-Induced Murine Model of Dilated Cardiomyopathy In Vivo
check_url/4016?article_type=v

Generation and Characterization of Right Ventricular Myocardial Infarction Induced by Permanent Ligation of the Right Coronary Artery in Mice

Read Article