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Chapters
- 00:05Title
- 01:00Dissection of the Cerebellum
- 02:05Processing the Tissue for Wholemount Staining
- 06:10Imaging the Stained Tissue
- 06:49Results: Zebrin II and CART Immunohistochemistry in Wholemount Cerebella
- 08:55Conclusion
Neurale kredsløb topografisk organiseret i funktionelle rum med specifikke molekylære profiler. Her giver vi de praktiske og tekniske skridt til at afsløre den globale hjerne topografi med et alsidigt wholemount immunhistokemisk farvning tilgang. Vi demonstrerer anvendeligheden af metoden under anvendelse af velforståede cytoarchitecture og kredsløb cerebellum.
Tags
Wholemount ImmunohistochemistryBrain TopographyCerebellumCellular ArchitectureGene Expression DomainsProtein Expression DomainsMolecular CompartmentalizationAfferent FibersPatterning DefectsMouse CerebellumWholemount Staining ApproachProtein Expression PatternsZebrinII/aldolaseC ExpressionThree-dimensional ConformationAfferent ProjectionsComparative StudiesMolecular TopographyRat Cerebellum
