A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 01:38Preparing the Animal for Sterile Surgery
- 02:53Delivering Tracers in vivo Using Sterile Surgery
- 05:31Neural Circuit Topography of the Cerebellum
- 08:31Conclusion
We provide a practical guide for delivering tracers in vivo and use the spinocerebellar pathway as a model system to demonstrate essential steps for successful neuronal circuit analysis in mice. We describe in detail our versatile tracing protocol that exploits wheat germ agglutinin (WGA) conjugated to Alexa fluorophores.
Tags
Neural Circuit TopographyMulti-color Neural CircuitsFunctional Topographic MapsVisualizing Circuit ArchitectureLabeling Topographic ProjectionsCerebellumSpinocerebellar Mossy FibersWheat Germ Agglutinin (WGA)Alexa 555Alexa 488Tracing Neural ProjectionsRetrograde TransportAnterograde TransportTracers PreparationSpinocerebellar Tracing SurgeryThree-dimensional ImagingFunctional Maps OrganizationConnectivity
