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1The Sidney Kimmel Comprehensive Cancer Center,The Johns Hopkins University School of Medicine, 2Department of Oncology,The Johns Hopkins University School of Medicine, 3Department of Dermatology,The Johns Hopkins University School of Medicine, 4Department of Surgery,Johns Hopkins University School of Medicine, 5The Sol Goldman Pancreatic Cancer Center,The Johns Hopkins University School of Medicine, 6Yale Cancer Center,Yale School of Medicine, 7The Skip Viragh Center for Pancreatic Cancer,The Johns Hopkins University School of Medicine, 8Department of Pathology,The Johns Hopkins University School of Medicine
Chapters
- 00:05Title
- 01:40Preparing Paraffin-embedded Slides
- 02:52Antigen Retrieval
- 03:39Staining
- 07:53Dehydration and Mounting
- 08:40Immunohistochemical Stains
- 09:39Conclusion
B7-H1 (PD-L1) and its binding to PD-1 provide a major tumor-induced immunosuppressive signal in the tumor’s microenvironment. An immunohistochemical staining technique to characterize the expression and localization of B7-H1 in pancreatic adenocarcinoma is described here.
Tags
Immunohistochemical StainingB7-H1PD-L1Paraffin-embedded SlidesImmune-regulatory Cell-surface ProteinsNegative RegulationCell-mediated Immune ResponsesReceptorProgrammed Death-1OverexpressionTumor CellsHuman CancersPrognostic MarkerAnti-tumor T-cell ImmunityTherapeutic TargetBlockade AntibodiesClinical TrialsSolid TumorsExpression And LocalizationPatient ResponseTreatment
