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High Throughput Single-cell and Multiple-cell Micro-encapsulation
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Bioengineering
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JoVE Journal Bioengineering
High Throughput Single-cell and Multiple-cell Micro-encapsulation

High Throughput Single-cell and Multiple-cell Micro-encapsulation

DOI:
10.3791/4096-v

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16:19 min

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June 15, 2012

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,
1Department of Mechanical Engineering,Vanderbilt University

Chapters

  • 00:05Title
  • 02:16Device Fabrication and Soft Lithography
  • 05:17Sample Preparation
  • 07:18Experimental Setup
  • 11:35Results: Controlled Single- and Double-particle Encapsulation
  • 15:44Conclusion

Summary

Automatic Translation

Automatic Translation

Combining monodisperse drop generation with inertial ordering of cells and particles, we describe a method to encapsulate a desired number of cells or particles in a single drop at kHz rates. We demonstrate efficiencies twice exceeding those of unordered encapsulation for single- and double-particle drops.

Tags

High ThroughputSingle-cellMultiple-cellMicro-encapsulationMicrofluidic Encapsulation MethodsPicoliter-scale DropsConfinementBulk Fluid EnvironmentHigh Throughput ScreeningCytometryMass SpectrometryEncapsulate Single CellsCapture Set Number Of CellsIsolationInteractions Between CellsControlled SizesDrop Generation TechniquesCell And Particle OrderingAqueous Particle SuspensionImmiscible Fluorocarbon OilFlow Focusing NozzleEncapsulation EfficiencyPolystyrene ParticlesPoisson Efficiencies

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