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Chapters
- 00:05Title
- 01:12Depleting Macrophages using Clodronate-containing Liposomes
- 02:15Derivation of Polarized Macrophages from Bone Marrow Aspirates
- 04:24Transferring Macrophages into Mice
- 05:17Results: Clodronate Liposome Depletion of Macrophages Allows Adoptive Transfer of Experimental Macrophage Populations
- 08:23Conclusion
Makrofager spiller en sentral rolle i homeostase og patologi i mange vev. Protokollen som presenteres her beskriver metoder for å tappe makrofager<em> In vivo</em>, Avledet polariserte makrofager fra benmargen aspirates, og adoptively overføre makrofager i mus. Disse teknikkene tillater bestemmelse av rollen som polariserte makrofager spille i helse og sykdom.
Tags
DepletionReconstitutionMacrophagesMiceInnate Immune ResponseAcquired Immune ResponseMacrophage DysfunctionDisease ProcessesInflammatory Bowel DiseasesPolarized PhenotypesClassically Activated MacrophagesAlternatively Activated MacrophagesIFN-gammaLPSIL-4IL-13InflammationInflammatory ResponseWhole Animal ModelDepleting MacrophagesComplex EnvironmentPhenotypically Defined Polarized MacrophagesBone Marrow Aspirates
