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Immunology and Infection

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JoVE Journal Immunology and Infection
Proteomic Profiling of Macrophages by 2D Electrophoresis
 

Proteomic Profiling of Macrophages by 2D Electrophoresis

Article DOI: 10.3791/52219-v • 07:53 min • November 4th, 2014
November 4th, 2014 •
1, 1, 1, 1, 1, 1, 1
1Inserm UMR744, University Lille Nord de France

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Summary
Automatic Translation

Macrophages are the key cells involved in host pathogenicity. Macrophages display phenotypic and functional diversity that can be analysed and detected by proteomic analysis. This article describes how to perform 2D electrophoresis of primary cultures of human macrophages differentiated into M1 or M2 phenotype.

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Keywords: Proteomic Profiling 2D Electrophoresis Macrophages M1 Phenotype M2 Phenotype DIGE Isoelectric Focusing Protein Expression Host Pathogenicity Microbial Toxins
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