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基于DNA载体的RNA干扰在癌症研究基因功能

JoVE Journal
Medicine

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JoVE Journal Medicine

DNA Vector-based RNA Interference to Study Gene Function in Cancer

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基于DNA载体的RNA干扰在癌症研究基因功能

DOI:

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13:10 min

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June 04, 2012

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Daniel B. Stovall, Meimei Wan, Qiang Zhang, Purnima Dubey, Guangchao Sui

¹Department of Cancer Biology and Comprehensive Cancer Center,Wake Forest University School of Medicine, ²Department of Pathology and Comprehensive Cancer Center,Wake Forest University School of Medicine

Chapters

00:05Title
01:54Generation of shRNA Constructs
04:33Lentivirus Transfection
06:35Infection and Characterization of Infected Cells
07:47Xenograft Mouse Model Study
10:39YY1 Knockdown Experiment
12:42Conclusion

Summary

Automatic Translation

RNA干扰（RNAi）拥有超过基因敲除的许多优点，在基因功能研究的工具已被广泛使用。基于DNA载体的RNAi技术的发明，已作出长期和诱导基因敲除可能，也增加了基因沉默的可行性<em>在体内</em>。

Tags

DNA Vector-based RNA Interference Gene Function Cancer Research SiRNA Gene Silencing RNAi Technology Small Hairpin RNA (shRNA)U6 Promoter H1 Promoter Lentivirus Lentiviral Vectors Cell Infection Mouse Xenograft Model ShRNA Constructs PCR Amplification

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