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Chapters
- 00:05Title
- 01:31Isolation of Mitochondria from Mouse Heart
- 04:40Quality Control: Measurement of Respiratory Control Ratio (RCR)
- 06:51Quality Control: Assessment of Mitochondrial Membrane Integrity (Cytochrome c Test)
- 07:34Measurement of the Mitochondrial Permeability Transition Pore Opening
- 10:03Representative Assay Results
- 13:08Conclusion
En spectrofluorometric protokoll for måling av mitokondrie permeabilitet overgangen pore åpningen i isolerte mus hjerte mitokondrier er presentert her. Analysen involverer samtidig måling av mitokondrier Ca<sup> 2 +</sup> Håndtering, mitokondrie membran potensial og mitokondrie volum. Fremgangsmåten for å oppnå høy kvalitet og funksjonell hjerte mitokondriene er også beskrevet.
Tags
Multi-parameter MeasurementPermeability Transition PoreIsolated Mouse Heart MitochondriaMitochondrial MembraneSolutesMolecular MassMtPTP FormationCyclophilin DVDACANTCell DeathMitochondrial Ca2+ HomeostasisBioenergeticsRedox SignalingMatrix Ca2+Oxidative StressAdenine Nucleotide DepletionHigh Concentrations Of PiMitochondrial Membrane DepolarizationUncouplingLong Chain Fatty AcidsIn VitroCalcium Retention CapacityProton Motive ForceMembrane Potential CollapseMitochondrial Matrix VolumeSwellingOuter Mitochondrial MembraneOrganelle FunctionFluorometric Measurement
