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셀 침습적 DISC1 단백질 종의 생성, 정제 및 특성
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Generation, Purification, and Characterization of Cell-invasive DISC1 Protein Species
DOI:

14:33 min

August 30, 2012

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Chapters

  • 00:05Title
  • 02:47Preparation of Aggresome Donor Cells: Transfection of mRFP- or eGFP-tagged Human Full-length DISC1
  • 03:50Aggresome Purification from Transfected Donor Cells
  • 06:43Treatment of Recipient SH-SYBY Cells with mRFP-DISC1 Aggresomes
  • 08:13Labeling of Recombinant DISC1(598-785) with DyLight594
  • 10:44Treatment of Recipient SH-SY5Y Cells with Labeled Recombinant DISC1(598-785) Protein
  • 12:00Representative Invasion Assay Results
  • 13:30Conclusion

Summary

Automatic Translation

배양 한 세포에서 세포, 세포질 전체 길이 DISC1 단백질 aggresomes와의 표시, multimeric 재조합 DISC1 단백질 조각의 생성, 정화 및 세포 침공<em> E. 대장균</em>에 설명되어 있습니다. 세포 invasiveness은 세포 배양과 뉴런에받는 사람 세포에 표시됩니다<em> 생체 내</em> stereotactical 뇌 접종 후.

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