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Chapters
- 00:05Title
- 01:28Generating Substrate: Polydimethylsiloxane (PDMS) Slides
- 02:49Generating Substrate: Polyacrylamide (PA) Slide
- 05:57Culturing Mammalian Cells on MEArrays for Functional Analysis
- 09:05Using MEArrays to Analyze How Type I Collagen and P-cadherin Affects Keratin Expression in Multipotent Mammary Epithelial Progenitor Cells
- 11:05Conclusion
Un metodo combinatorio screening funzionale per ottenere approfondimenti sugli impatti della composizione molecolare di microambienti su funzioni cellulari è descritto. Il metodo si avvale di microarray esistenti basate su tecnologie per generare matrici di microambienti definite combinatorie che supportano l'adesione cellulare e analisi funzionale.
Tags
FabricationUseMicroEnvironment MicroArraysMEArraysInteractionsCellsMicroenvironmentFunctional ConsequencesSingle Cell LevelDifferentiationMigrationProliferation PhenotypesMorphogenesisTumorigenesisCell-cell InteractionsECM InteractionsSoluble Factor InteractionsPhysical CharacteristicsGenomic ContentCombinatorial InteractionsMicroenvironmental ComponentsDistinctive BehaviorsMEArray PlatformCell-based Functional ScreeningMolecular CompositionElastic ModulusMicroarray TechnologyMicropatterning Technology
