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抗体转染神经元作为一种工具来研究疾病的发病机制
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Antibody Transfection into Neurons as a Tool to Study Disease Pathogenesis
DOI:

06:56 min

September 26, 2012

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Chapters

  • 00:05Title
  • 01:17Neuronal Cell Culture
  • 01:39Antibody Labeling
  • 02:48Antibody Transfection
  • 04:18Live and Fixed Imaging to Determine Transfection Efficiency
  • 05:11Results: Live and Fixed Imaging of Antibody-transfected Neurons
  • 05:52Conclusion

Summary

Automatic Translation

一种快速的方法,调查相关的抗体的存在下,在细胞内环境中的分子机制的相互作用和效果进行说明。该方法涉及的抗体的转染到活细胞,使用的基础上的脂质制剂的非共价复合物的形成。该技术是适应的永生化细胞系和原代细胞。

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