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一个的趋电含量为神经前体细胞迁移的动力学分析外部施加直流电场

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

A Galvanotaxis Assay for Analysis of Neural Precursor Cell Migration Kinetics in an Externally Applied Direct Current Electric Field

Please note that all translations are automatically generated. Click here for the English version.

一个的趋电含量为神经前体细胞迁移的动力学分析外部施加直流电场

DOI:

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11:00 min

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October 13, 2012

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Robart Babona-Pilipos, Milos R. Popovic, Cindi M. Morshead

¹Institute for Biomaterials and Biomedical Engineering,University of Toronto, ²Lyndhurst Centre,Toronto Rehabilitation Institute, ³Department of Surgery,University of Toronto

Chapters

00:05Title
01:25Galvanotaxis Chamber Preparation
05:30Live Cell Time-lapse Imaging
08:55Kinematic and IHC Analysis of Differentiated and Undifferentiated NPCs
10:35Conclusion

Summary

Automatic Translation

在这个协议中，我们将演示如何构建自定义室，允许应用程序的直流电场，使时间的推移成像的成人脑源性神经前体细胞转趋电性。

Tags

Galvanotaxis Assay Neural Precursor Cell Migration Externally Applied Direct Current Electric Field Neural Stem Cells Neuroregenerative Strategies Mobilization Of NPCs Lesion Site Endogenous Precursors Periventricular Region Mechanisms Of NPC Migration Direct Current Electric Fields (dcEFs)Galvanotaxis Phenomenon Physiological Electric Fields Cell Migration Normal Development Wound Repair

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