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Chapters
- 00:05Title
- 02:03Haploid Cell Growth
- 03:08Conducting a Cross
- 04:43Harvesting Preparations
- 05:57Flow Cytometry
- 09:01Preparation for DeltaVision Microscopy
- 11:05Representative Analysis of Zygote Purification
- 14:27Conclusion
For at oprense zygoter af<em> S. cerevisiae</em>, Blev haploide celler af modsat parringstype manipuleret til at udtrykke rødt eller grønt fluorescerende proteiner, co-inkuberede at tillade zygote formation og fraktioneret under anvendelse af en flow-cytometri-baseret protokol. Det stærkt fraktion muliggør efterfølgende "-miske" undersøgelser, inddrivelse af initial afkom, og systematisk undersøgelse af zygote morfogenese.
Tags
Flow Cytometry-based PurificationS. Cerevisiae ZygotesHaploid CellsDiploid StatesMating TypeStable DiploidsMitotic DivisionsZygote FormationSoluble Mating FactorsReceptor-mediated Signaling CascadesCell-cell FusionModel For Progenitor Or Stem Cell FunctionBiochemistry Of Zygote FormationPurification Of Yeast ZygotesSedimentation PropertiesHypertonic Sorbitol
