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Chapters
- 00:05Title
- 01:47Extraction of Glycosphingolipids from Rat Leukemia Cells
- 02:50Separation of Neutral and Acidic Lipids
- 05:03Removal of Phospholipids from the Neutral Fraction
- 08:14Permethylation of Glycosphingolipids
- 10:04Results: Mass Spectra of Permethylated Glycosphingolipids
- 12:12Conclusion
En specifik og følsom metode til at få indsigt i udtrykket profil glycosphingolipid antigener i immune organer og celler er beskrevet. Fremgangsmåden drager fordel af den ion-trap massespektrometri tillader trinvis fragmentering af glycosphingolipid molekyler til strukturel analyse i sammenligning med kemisk syntetiserede standarder.
Tags
Mass Spectrometric AnalysisGlycosphingolipid AntigensGSL’sGlycoconjugate BiomacromoleculesStructural InformationEmbryonic DevelopmentSignal TransductionImmune Receptor RecognitionSugar MoietiesIsomersLipid MoietiesFatty Acyl Chain LengthUnsaturationHydroxylationCarbohydrate PortionCeramide PortionTri-hexosylceramidesGlobotriaosylceramide (Galα4Galβ4Glcβ1Cer)Isoglobotriaosylceramide (Galα3Galβ4Glcβ1Cer)Molecular MassesSugar LinkagesBiological FunctionsModification Of Ceramide PartAlpha-galactosylceramideCytokine Secretion ProfilesInvariant NKT CellsCancer ModelsAutoimmune DiseasesStructural Analysis Of IsomersImmune Organs And Cells
