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紧凑型量子点的单分子成像
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JoVE Journal Engineering
Compact Quantum Dots for Single-molecule Imaging
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紧凑型量子点的单分子成像

DOI:
10.3791/4236-v

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17:14 min

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October 09, 2012

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1Department of Biomedical Engineering,Emory University, 2Department of Chemistry,Georgia Institute of Technology

Chapters

  • 00:05Title
  • 01:52Synthesis of Mercury Cadmium Selenide (HgxCd1-xSe) Quantum Dot Cores
  • 06:49Growth of Cadmium Zinc Sulfide (CdyZn1-yS) Shell
  • 09:20Phase Transfer
  • 12:01PEG Coating
  • 13:10Results: Quantum Dot Characterization
  • 16:39Conclusion

Summary

Automatic Translation

Automatic Translation

我们描述了准备的胶体量子点与最小化的流体力学尺寸为单分子荧光成像。相比传统的量子点，这些纳米颗粒的尺寸类似于球状蛋白质单分子反对光降解，亮度，稳定性和耐非特异性结合的蛋白质和细胞进行了优化。

Tags

Compact Quantum DotsSingle-molecule ImagingBiomolecular FunctionSpatial HeterogeneityTemporal HeterogeneityEpifluorescence MicroscopyTotal Internal Reflection Fluorescence MicroscopyFluorescent TagDyeProteinBeadQuantum DotHigh Photon FluxesFluorescence ImagingSignal StabilityHydrodynamic SizeDiffusion BehaviorSemiconductor MaterialsPhotodegradation

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