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病毒遗传背景明确的神经回路跟踪
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Neuroscience
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JoVE Journal Neuroscience
Viral Tracing of Genetically Defined Neural Circuitry
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病毒遗传背景明确的神经回路跟踪

DOI:
10.3791/4253-v

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13:06 min

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October 17, 2012

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,
1Department of Genetics,Harvard Medical School, 2Howard Hughes Medical Institute,Harvard Medical School

Chapters

  • 00:05Title
  • 01:16Passage and Concentration of Vesicular Stomatitis Virus (VSV)
  • 04:23Stereotaxic Injection of Virus
  • 08:19Tissue Harvest and Preparation for Microscopic Analysis
  • 09:41Results: Behavior of rVSV(A/RG) Virus in Various Cell Types
  • 12:22Conclusion

Summary

Automatic Translation

Automatic Translation

跟踪突触连接的神经元的方法。我们使用TVA特异性的上游电池，探测是否感兴趣的接收的细胞群体突触输入从基因定义的细胞类型。

Tags

Viral TracingGenetically Defined Neural CircuitryTranssynaptic VirusesPseudorabies VirusRabies VirusVesicular Stomatitis VirusAnterograde DirectionRetrograde DirectionMonosynaptic Retrograde TracingGlycoprotein GeneChimeric GTVA ReceptorInputs Onto A Defined Cell TypeVSV As A Transsynaptic Tracer

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