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Spændingsfølsomme Dye Optagelse fra axoner, dendritter og dendrit udløberne af individuelle neuroner i hjernen Slices
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Chapters
- 00:05Title
- 02:09Equipment Setup
- 04:35Whole-cell Patch-clamp Recording and Dye Diffusion
- 07:35Optical Recording
- 09:30Results: Voltage-sensitive Dye Recording from Axons, Dendrites and Dendritic Spines of Individual Neurons in Brain Slices
- 11:54Conclusion
En imaging teknik til overvågning af membran potentielle ændringer i sub-mikrometer rumlige og sub-millisekund tidsopløsning er beskrevet. Teknikken, baseret på laser excitation af spændingsfølsomme farvestoffer, tillader målinger af signaler i axoner og axon soeskende terminale dendritiske grene, og individuelle dendritiske spidser.
Tags
Voltage-sensitive Dye RecordingAxonsDendritesDendritic SpinesIndividual NeuronsBrain SlicesBiophysical PropertiesFunctional OrganizationElectrical SignalsNerve CellElectrical PropertiesMeasurementsSubthreshold EventsAction Potential InitiationPatch-electrode ApproachImaging TechniquesParallel RecordingsMembrane Potential TransientsOrganic Voltage-sensitive Dyes (Vm-imaging)Sub-millisecond ResolutionSub-micrometer ResolutionVoltage-sensitive Molecular Probes
