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JoVE Journal Neuroscience
Recording Electrical Activity from Identified Neurons in the Intact Brain of Transgenic Fish
 

Recording Electrical Activity from Identified Neurons in the Intact Brain of Transgenic Fish

Article DOI: 10.3791/50312-v • 07:45 min • April 30th, 2013
April 30th, 2013 •
1, 1
1Department of Physiology, University of California, Los Angeles

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In this video, we will demonstrate how to record electrical activity from identified single neurons in a whole brain preparation, which preserves complex neural circuits. We use transgenic fish in which gonadotropin-releasing hormone (GnRH) neurons are genetically tagged with a fluorescent protein for identification in the intact brain preparation.

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Keywords: Transgenic Fish GnRH Neurons Medaka Fish Terminal Nerve Neuromodulatory Spontaneous Action Potential Firing Kisspeptin Intact Brain Neuron Identification Electrophysiology
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