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Patch clamp optagelser i indre øre-hårceller isoleret fra zebrafisk
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Patch Clamp Recordings in Inner Ear Hair Cells Isolated from Zebrafish

Patch clamp optagelser i indre øre-hårceller isoleret fra zebrafisk

DOI:
10.3791/4281-v

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12:43 min

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October 17, 2012

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, , , , , , ,
1Natural Science Division,Pepperdine University

Chapters

  • 00:05Title
  • 01:52Pre-experimental Preparations
  • 02:47Isolation of Auditory and Vestibular Labyrinth
  • 05:28Hair Cell Isolation
  • 07:18Patch Clamping Zebrafish Hair Cells
  • 10:12Results: Isolation of and Electrophysiological Recordings from Zebrafish Hair Cells
  • 12:04Conclusion

Summary

Automatic Translation

Automatic Translation

Formålet med denne video er at demonstrere procedurer til opnåelse af raske, intakte hårceller fra det indre øre organer voksne zebrafisk og derefter bruge dem til patch clamp-forsøg med henblik på at karakterisere de biofysiske egenskaber af de spændingsstyrede kanaler.

Tags

Patch Clamp RecordingsInner Ear Hair CellsZebrafishVoltage-gated ChannelsSensory Hair CellsIsolation MethodHair Cell SizeHair Cell MorphologyDiversityPatch Clamp RecordingsZebrafish Model SystemMutantsHearing And BalanceTransgenic LinesGenetic AnalysisElectrophysiological MethodsSensory Modalities

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