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Journal / Neuroscience / Whole-cell Patch-clamp Recordings in Brain Slices
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JoVE Journal Neuroscience
Whole-cell Patch-clamp Recordings in Brain Slices
 

Whole-cell Patch-clamp Recordings in Brain Slices

Article DOI: 10.3791/54024-v • 07:23 min • June 15th, 2016
June 15th, 2016 •
1, 1, 1
1Department of Psychiatry, University of Texas Southwestern Medical Center

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This protocol describes basic procedural steps for performing whole-cell patch-clamp recordings. This technique allows the study of the electrical behavior of neurons, and when performed in brain slices, allows the assessment of various neuronal functions from neurons that are still integrated in relatively well preserved brain circuits.

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Keywords: Whole-cell Patch-clamp Brain Slices Neuroscience Neuron Function In Vitro In Vivo Recording Chamber ACSF Micropipette Internal Solution Electrode Holder Micromanipulator
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