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使用荧光显微镜可视化中的细菌线虫
JoVE Journal
Biology
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JoVE Journal Biology
Visualizing Bacteria in Nematodes using Fluorescent Microscopy
DOI:

09:02 min

October 19, 2012

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Chapters

  • 00:05Title
  • 00:56Construction of a Fluorescent Bacterial Strain via Conjugation
  • 02:22Production of Axenic Nematode Eggs
  • 04:45Co-cultivation Assay with Fluorescent Bacteria
  • 05:52Collection and Screening of Nematodes for Bacterial Association by Microscopy
  • 06:42Results: Representative Nematode and Bacteria Imagery
  • 08:33Conclusion

Summary

Automatic Translation

要研究之间的互利共生<em>嗜</em细菌和<em>斯氏线虫</em监测细菌的存在和位置内线虫线虫的方法被开发。实验的方法,它可以应用于其它系统,带来工程菌表达绿色荧光蛋白和可视化,使用荧光显微镜细菌内的透明的线虫。

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