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Chapters
- 00:05Title
- 01:34Engineering of Cells with Visible Plasmids
- 05:08Visualization of Labeled DNA
- 09:33Post-acquisition Processing of Images
- 10:33Results: Visualization of Plasmids in Live Mammalian Cells
- 12:16Conclusion
En fremgangsmåde til at observere de individuelle DNA-molekyler i levende celler er beskrevet. Teknikken er baseret på binding af en fluorescens-mærket lac repressorproteinet til bindingssteder splejset ind DNA'et af interesse. Denne fremgangsmåde kan tilpasses til at følge mange rekombinante DNA'er i levende celler over tid.
Tags
Plasmid ReplicationLive Mammalian CellsFluorescence MicroscopyGamma-herpesvirusesEpstein-Barr VirusKaposi’s Sarcoma-associated HerpesvirusLicensed ReplicationViral ProteinCellular Replication MachineryCentromeresSouthern BlottingFluorescence In Situ Hybridization (FISH)Quantitative PCRVisualizing PlasmidsLactose Repressor ProteinLacO Sequence
