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Simultaneous Measurement of Mitochondrial Calcium and Mitochondrial Membrane Potential in Live Cells by Fluorescent Microscopy
 

Simultaneous Measurement of Mitochondrial Calcium and Mitochondrial Membrane Potential in Live Cells by Fluorescent Microscopy

Article DOI: 10.3791/55166-v • 08:43 min • January 24th, 2017
January 24th, 2017 •
1,2, 1, 3
1Centre for Genetic Diseases, Hudson Institute of Medical Research, 2The Department of Molecular and Translational Sciences, Monash University, 3Department of Physiology, University College London

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Summary
Automatic Translation

Mitochondria can utilize the electrochemical potential across their inner membrane (ΔΨm) to sequester calcium (Ca2+), allowing them to shape cytosolic Ca2+ signaling within the cell. We describe a method for simultaneously measuring mitochondria Ca2+ uptake and ΔΨm in live cells using fluorescent dyes and confocal microscopy.

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Keywords: Mitochondrial Calcium Mitochondrial Membrane Potential Fluorescent Microscopy Live Cell Imaging Calcium Regulation Mitochondrial Disease Calcium-free HBSS TMRM Fluo 4 AM Digitonin Thapsigargin
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