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体外实时成像在小鼠神经上皮单细胞分裂
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Neuroscience
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JoVE Journal Neuroscience
Ex vivo Live Imaging of Single Cell Divisions in Mouse Neuroepithelium
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体外实时成像在小鼠神经上皮单细胞分裂

DOI:
10.3791/4439-v

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06:41 min

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April 30, 2013

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,
1Department of Human Genetics,Emory University School of Medicine, 2Department of Experimental Embryology,IGAB Polish Academy of Sciences

Chapters

  • 00:05Title
  • 01:13Embryo Generation
  • 02:09Whole Mouse Embryo Culture and Viral Infection
  • 03:27Neural Tube Slice Preparation and Live Imaging
  • 05:14Representative Live Imaging Results
  • 06:20Conclusion

Summary

Automatic Translation

Automatic Translation

在这里，我们开发所需的工具<em>体外</em>实时成像跟踪单E8.5的小鼠神经上皮细胞分裂

Tags

Ex Vivo Live ImagingSingle Cell DivisionsMouse NeuroepitheliumFluorescent MarkersCulturing SlicesEmbryonic Mouse NeuroepitheliumGenetic Cell Lineage TracingTamoxifen-inducible Cre LineCre Reporter LineDsRed ReporterCre-mediated RecombinationTamoxifen InductionIndividual Cell DivisionsTranscriptional ResponseSonic Hedgehog SignalingOlig2-eGFP Transgenic LineCilia MarkerSstr3-GFP ReporterNeuroepithelium ImagingTime-lapse Confocal ImagingPrimary Cilia FormationShh Response

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