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利用双光子显微镜在慕尼黑Wistar大鼠量化肾小球通透性荧光大分子
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JoVE Journal
Medicine
Quantifying Glomerular Permeability of Fluorescent Macromolecules Using 2-Photon Microscopy in Munich Wistar Rats
Please note that all translations are automatically generated.
Click here for the English version.
利用双光子显微镜在慕尼黑Wistar大鼠量化肾小球通透性荧光大分子
DOI:
10.3791/50052-v
•
11:13 min
•
April 17, 2013
•
Ruben M. Sandoval
,
Bruce A. Molitoris
1
Medicine/Nephrology
,
Indiana University School of Medicine
Chapters
00:05
Title
01:50
Exposing the Kidney in a Munich Wistar Rat for Intravital 2-Photon Imaging
04:10
Acquiring Images to Quantify Renal Permeability of Albumin
06:02
Calculating Glomerular Sieving Coefficient for Fluorescent Albumin from 3D Volumes
08:21
Glomerular Permeability Results
10:34
Conclusion
Summary
Automatic Translation
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Automatic Translation
利用高分辨率intavital 2 - 光子显微镜的技术直接可视化和量化表面肾小球过滤gloemrular。此方法允许在正常和疾病状态的大分子渗透特性的直接测定。
Tags
2-photon Microscopy
Glomerular Permeability
Fluorescent Macromolecules
Munich Wistar Rats
Kidney Diseases
Urinary Loss
Serum Albumin
Podocytes
Vascular Endothelial Cells
Basement Membrane
Glomerular Filtration Barrier
Proximal Tubule Cells
Micropuncture
Dextran Polymers
Bowman’s Space
Albumin Reabsorption
Tubular Transcytosis
Proteinuric Renal Diseases
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