/
/
Generation og indvinding af β-celle Sfæroider Fra Step-vækst PEG-peptid hydrogeler
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 02:01Material Preparation and Sterilization
- 03:26Cell Preparation
- 04:46Hydrogel Fabrication and Cell Encapsulation
- 05:57Chymotrypsin Mediated Gel Erosion and Spheroid Recovery
- 07:24Results: Spheroid Encapsulation, Survival, Proliferation, and Recovery in Thiol-ene Hydrogels
- 08:31Conclusion
Den følgende protokol giver teknikker til indkapsling af bugspytkirtlen β-celler i trin-vækst PEG-peptid hydrogeler dannet af thiol-foto-klik reaktioner. Dette materiale platform tilbyder ikke kun en cytocompatible mikromiljø for celleindkapsling, men også tillader brugeren kontrolleret, hurtig genopretning af cellestrukturer dannet inden for hydrogeler.
