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Generation and Recovery of β-cell Spheroids From Step-growth PEG-peptide Hydrogels
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Chapters
- 00:05Title
- 02:01Material Preparation and Sterilization
- 03:26Cell Preparation
- 04:46Hydrogel Fabrication and Cell Encapsulation
- 05:57Chymotrypsin Mediated Gel Erosion and Spheroid Recovery
- 07:24Results: Spheroid Encapsulation, Survival, Proliferation, and Recovery in Thiol-ene Hydrogels
- 08:31Conclusion
The following protocol provides techniques for encapsulating pancreatic β-cells in step-growth PEG-peptide hydrogels formed by thiol-ene photo-click reactions. This material platform not only offers a cytocompatible microenvironment for cell encapsulation, but also permits user-controlled rapid recovery of cell structures formed within the hydrogels.
