Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Medicine / Screening for Melanoma Modifiers using a…
JoVE Journal
Medicine

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Medicine
Screening for Melanoma Modifiers using a Zebrafish Autochthonous Tumor Model
 

Screening for Melanoma Modifiers using a Zebrafish Autochthonous Tumor Model

Article DOI: 10.3791/50086-v • 10:23 min • November 13th, 2012
November 13th, 2012 •
1, 2,3, 1
1Program in Molecular Medicine and Department of Cancer Biology, University of Massachusetts Medical School, 2Departments of Surgery and Medicine, Weill Cornell Medical College, 3Departments of Surgery and Medicine, New York Presbyterian Hospital

Chapters

Summary
Automatic Translation

A rapid way to screen for melanoma modifiers using a zebrafish autochthonous tumor model is presented. It takes advantage of the miniCoopR vector which allows for expression of candidate melanoma genes in melanocytes. A method to obtain melanoma-free survival curves, an invasion assay, a protocol for antibody staining of scale melanocytes and a melanoma transplantation assay are described.

Tags

Keywords: Melanoma Zebrafish Autochthonous Tumor Model Genomic Studies Melanoma Modifiers MiniCoopR Vector Mitfa BRAFV600E P53 Transgenesis Melanoma Initiation Melanoma Cell Properties
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter