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目标移植的肌肉祖细胞的分子成像

JoVE Journal
Medicine

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JoVE Journal Medicine

Molecular Imaging to Target Transplanted Muscle Progenitor Cells

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目标移植的肌肉祖细胞的分子成像

DOI:

10.3791/50119-v

•

09:24 min

•

March 27, 2013

•

Kelly Gutpell², Rebecca McGirr, Lisa Hoffman^2,3

¹Imaging Program,Lawson Health Research Institute, ²Department of Anatomy and Cell Biology,Western University, ³Department of Medical Biophysics,Western University

Chapters

00:05Title
02:17Culture and Transfection of C2C12 Myoblasts
04:26Preparation of Myoblasts for Transplant
05:16Cell Implantation
06:49Injection of the Fluorescent Substrate
07:13Bioluminescence Imaging to Image Luciferase-Expressing Myoblast Progenitor Cells
07:37Results: Molecular Imaging to Target Transplanted Muscle Progenitor Cells
08:42Conclusion

Summary

Automatic Translation

非侵入性的方法来评估成肌细胞移植的成功。该方法利用一个统一的融合的记者基因组成的基因的表达，可以被不同的成像方式成像。在这里，我们使用<em>的波动</em>报告基因序列到靶细胞通过生物发光成像。

Tags

Keywords: Molecular Imaging Muscle Progenitor Cells Duchenne Muscular Dystrophy Bioluminescence Imaging PET SPECT MRI Ultrasound Reporter Gene C2C12 Myoblasts Mdx Mouse

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