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Chapters
- 00:05Title
- 02:17Culture and Transfection of C2C12 Myoblasts
- 04:26Preparation of Myoblasts for Transplant
- 05:16Cell Implantation
- 06:49Injection of the Fluorescent Substrate
- 07:13Bioluminescence Imaging to Image Luciferase-Expressing Myoblast Progenitor Cells
- 07:37Results: Molecular Imaging to Target Transplanted Muscle Progenitor Cells
- 08:42Conclusion
En ikke-invasiv måde at evaluere succes myoblast transplantation er beskrevet. Fremgangsmåden drager fordel af en samlet fusion reportergen bestående af gener, hvis ekspression kan afbildes med forskellige billeddannende modaliteter. Her vil vi gøre brug af en<em> Udsving</em> Reportergen sekvens til målceller via bioluminescens billeddannelse.
