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JoVE Journal Neuroscience
Generation of Topically Transgenic Rats by In utero Electroporation and In vivo Bioluminescence Screening
 

Generation of Topically Transgenic Rats by In utero Electroporation and In vivo Bioluminescence Screening

Article DOI: 10.3791/50146-v • 08:44 min • September 24th, 2013
September 24th, 2013 •
1, 2, 2, 3, 1
1Department of Neuropathology, Medical School Düsseldorf, 2Department of Molecular Genetics, Weizmann Institute for Science, 3Center of Behavioral Neuroscience, University of Düsseldorf

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Summary
Automatic Translation

Genes can be manipulated during development of the cortex or the hippocampus of the rat via in utero electroporation (IUE) at E16, to enable fast and targeted modifications in neuronal connectivity for later studies of behavior or neuropathology in adult animals. Postnatal in vivo imaging for control of IUE success is performed by bioluminescence of activating co-transfected luciferase.

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Keywords: In Utero Electroporation Bioluminescence Imaging Transgenic Rats DISC1 Neurodevelopment Behavioral Neuroscience
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