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分离新生小鼠心肌文化和
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JoVE Journal
Biology
Isolation and Culture of Neonatal Mouse Cardiomyocytes
Please note that all translations are automatically generated.
Click here for the English version.
分离新生小鼠心肌文化和
DOI:
10.3791/50154-v
•
07:50 min
•
September 06, 2013
•
Elisabeth Ehler
,
Thomas Moore-Morris
,
Stephan Lange
1
Randall and Cardiovascular Divisions
,
King’s College London
,
2
Division of Cardiology, School of Medicine
,
University of California San Diego
Chapters
00:05
Title
01:33
Day 1: Isolation of the Cardiac Tissue from Neonatal Mice
02:53
Day 2: Enzymatic Tissue Digestion and Plating of Cells
05:29
Day 3: Culture of Neonatal Cardiomyocytes
06:01
Results: Culture of Neonatal Mouse Cardiomyocytes and Representative Immunofluorescent Images
07:03
Conclusion
Summary
Automatic Translation
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Automatic Translation
原代小鼠心肌细胞培养物的肌原纤维组织和功能的研究的关键工具之一。以下协议描述了从新生小鼠心脏原发性心肌细胞的分离和培养。所得到的心肌细胞培养物随后可用于各种生物力学,生物化学和细胞生物学测定。
Tags
Neonatal Mouse Cardiomyocytes
Myofibrillogenesis
Myofibrillar Functions
Cell Isolation
Cell Culture
Enzymatic Dissociation
Cell Viability
Biochemical Assays
Electrophysiology
Biomechanics
Protocol Optimization
Reproducibility
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