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分离新生小鼠心肌文化和

JoVE Journal
Biology

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JoVE Journal Biology

Isolation and Culture of Neonatal Mouse Cardiomyocytes

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分离新生小鼠心肌文化和

DOI:

10.3791/50154-v

•

07:50 min

•

September 06, 2013

•

Elisabeth Ehler, Thomas Moore-Morris, Stephan Lange

¹Randall and Cardiovascular Divisions,King’s College London, ²Division of Cardiology, School of Medicine,University of California San Diego

Chapters

00:05Title
01:33Day 1: Isolation of the Cardiac Tissue from Neonatal Mice
02:53Day 2: Enzymatic Tissue Digestion and Plating of Cells
05:29Day 3: Culture of Neonatal Cardiomyocytes
06:01Results: Culture of Neonatal Mouse Cardiomyocytes and Representative Immunofluorescent Images
07:03Conclusion

Summary

Automatic Translation

原代小鼠心肌细胞培养物的肌原纤维组织和功能的研究的关键工具之一。以下协议描述了从新生小鼠心脏原发性心肌细胞的分离和培养。所得到的心肌细胞培养物随后可用于各种生物力学，生物化学和细胞生物学测定。

Tags

Neonatal Mouse Cardiomyocytes Myofibrillogenesis Myofibrillar Functions Cell Isolation Cell Culture Enzymatic Dissociation Cell Viability Biochemical Assays Electrophysiology Biomechanics Protocol Optimization Reproducibility

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