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Identifikation af<em> Tornerose</em> Transposon Insertioner i solide tumorer med Linker-medieret PCR
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JoVE Journal Medicine
Identification of Sleeping Beauty Transposon Insertions in Solid Tumors using Linker-mediated PCR

Identifikation af<em> Tornerose</em> Transposon Insertioner i solide tumorer med Linker-medieret PCR

DOI:
10.3791/50156-v

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10:34 min

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February 01, 2013

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1Department of Obstetrics, Gynecology & Women’s Health, Masonic Cancer Center,University of Minnesota, Minneapolis, 2Department of Genetics, Cell Biology & Development, Center for Genome Engineering,University of Minnesota, Minneapolis

Chapters

  • 00:05Title
  • 01:00Tumor Collection and DNA Isolation
  • 02:16Digesting and Ligating Linkers to the DNA
  • 05:51Removing Concatamer Transposons Using a Second Digestion
  • 06:54Amplifying the Tumor DNA
  • 09:19PCR and Sequencing Results
  • 10:08Conclusion

Summary

Automatic Translation

Automatic Translation

En fremgangsmåde til identifikation af ukendte førere af carcinogenese under anvendelse af en objektiv metode er beskrevet. Fremgangsmåden anvender<em> Tornerose</em> Transposon som en tilfældig mutagen rettet mod specifikke væv. Genomisk kortlægning af transposon-insertioner, der driver tumordannelse identificerer hidtil ukendte onkogener og tumorsuppressorgener

Tags

Sleeping Beauty TransposonForward Genetic ScreenLinker-mediated PCRTumor GeneticsCancer GenomeGenetic DriversPassenger MutationsGenetic HeterogeneityCre RecombinaseTransposaseCommon Insertion Sites

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