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(TET-ON)与四环素诱导shRNA或cDNA表达生成稳定的人类细胞系
JoVE Journal
Biology
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JoVE Journal Biology
Generation of Stable Human Cell Lines with Tetracycline-inducible (Tet-on) shRNA or cDNA Expression
DOI:

09:51 min

March 05, 2013

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Chapters

  • 00:05Title
  • 01:22Generation of Cell Lines Stably Expressing TetR
  • 04:51pTER and pT-Rex DEST30: shRNA or cDNAs Expression
  • 05:30Stable Cell Lines with Tetracycline-inducible Expression
  • 08:31Results: Stable Human Tet-on Cell Lines
  • 09:27Conclusion

Summary

Automatic Translation

一种快速简便的方法来产生人类细胞系诱导的,可逆的基因表达或shRNA介导的,感兴趣的基因敲除。此方法使研究人员能够可靠地和高再现性操作的细胞系,是很难改变,通过瞬时转染的方法或常规的击倒/基因敲除策略。

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