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Generation of Stable Human Cell Lines with Tetracycline-inducible (Tet-on) shRNA or cDNA Expression
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Generation of Stable Human Cell Lines with Tetracycline-inducible (Tet-on) shRNA or cDNA Expression

Generation of Stable Human Cell Lines with Tetracycline-inducible (Tet-on) shRNA or cDNA Expression

DOI:
10.3791/50171-v

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09:51 min

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March 05, 2013

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, ,
1UCL Cancer Institute, 2Friedrich Miescher Institute for Biomedical Research

Chapters

  • 00:05Title
  • 01:22Generation of Cell Lines Stably Expressing TetR
  • 04:51pTER and pT-Rex DEST30: shRNA or cDNAs Expression
  • 05:30Stable Cell Lines with Tetracycline-inducible Expression
  • 08:31Results: Stable Human Tet-on Cell Lines
  • 09:27Conclusion

Summary

Automatic Translation

Automatic Translation

A rapid and simple way to generate human cell lines with inducible and reversible cDNA overexpression or shRNA-mediated knock-down of the gene of interest. This method enables researchers to reliably and highly reproducibly manipulate cell lines that are difficult to alter by transient transfection methods or conventional knockdown/knockout strategies.

Tags

Keywords: Tetracycline-inducibleTet-onShRNACDNA ExpressionGene ExpressionMammalian Cell LinesRNA InterferenceGene KnockdownGene OverexpressionTet-off SystemTet-on SystemTetRTet-operatorCell BiologyGene FunctionGene Manipulation

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