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高分子基因传递纳米粒子的纳米粒子跟踪分析和高通量流式细胞仪评价
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry
DOI:

08:51 min

March 01, 2013

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Chapters

  • 00:05Title
  • 01:21Cell Seeding and Transfection
  • 04:34Nanoparticle Sizing and Plasmid per Particle Calculations
  • 06:37Representative Data
  • 08:01Conclusion

Summary

Automatic Translation

纳米粒子跟踪分析(NTA)和高通量流式细胞仪来评价聚合物纳米基因传递的协议。 NTA用于表征的纳米粒子的粒径分布和每个粒子分布的质粒。高通量的基因传递的生物材料库,流式细胞仪,定量的转染效率评价。

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