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Bioengineering
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高分子基因传递纳米粒子的纳米粒子跟踪分析和高通量流式细胞仪评价
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry
Please note that all translations are automatically generated.
Click here for the English version.
高分子基因传递纳米粒子的纳米粒子跟踪分析和高通量流式细胞仪评价
DOI:
10.3791/50176-v
•
08:51 min
•
March 01, 2013
•
Ron B. Shmueli
2
,
Nupura S. Bhise
2
,
Jordan J. Green
2,3,4
1
Biomedical Engineering Department
,
Johns Hopkins University School of Medicine
,
2
Translational Tissue Engineering Center
,
Johns Hopkins University School of Medicine
,
3
Wilmer Eye Institute
,
Johns Hopkins University School of Medicine
,
4
Institute for Nanobiotechnology
,
Johns Hopkins University School of Medicine
Chapters
00:05
Title
01:21
Cell Seeding and Transfection
04:34
Nanoparticle Sizing and Plasmid per Particle Calculations
06:37
Representative Data
08:01
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
纳米粒子跟踪分析(NTA)和高通量流式细胞仪来评价聚合物纳米基因传递的协议。 NTA用于表征的纳米粒子的粒径分布和每个粒子分布的质粒。高通量的基因传递的生物材料库,流式细胞仪,定量的转染效率评价。
Tags
Polymeric Gene Delivery
Nanoparticle Tracking Analysis
High-throughput Flow Cytometry
Non-viral Gene Delivery
Cationic Polymers
Polylysine
Polyphosphoesters
Poly(amidoamines)
Polyethylenimine (PEI)
Poly(beta-amino Ester)s (PBAEs)
Human Retinal Endothelial Cells (HRECs)
Transfection Efficiency
96-well Plate Assay
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