Medición de transporte de cationes por Na, K y H, K-ATPasa en<em> Xenopus</em> Los ovocitos mediante espectrofotometría de absorción atómica: una alternativa a ensayos de radioisótopos
We describe a method to quantify the activity of K+-countertransporting P-type ATPases by heterologous expression of the enzymes in Xenopus oocytes and measuring Rb+ or Li+ uptake into individual cells by atomic absorption spectrophotometry. The method is a sensitive and safe alternative to radioisotope flux experiments facilitating complex kinetic studies.