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染色体制备培养细胞

JoVE Journal
Biology

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JoVE Journal Biology

Chromosome Preparation From Cultured Cells

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染色体制备培养细胞

DOI:

10.3791/50203-v

•

07:42 min

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January 28, 2014

•

Bradley Howe, Ayesha Umrigar, Fern Tsien

¹Department of Genetics,Louisiana State University Health Science Center

Chapters

00:05Title
01:41Chromosome Harvesting of Adherent Cells
03:52Slide preparation and Solid Staining
05:35Banding Using Trypsin and Giemsa (GTG)
06:44Results: Chromosome Spreads
07:02Conclusion

Summary

Automatic Translation

染色体可以从活细胞如淋巴细胞或皮肤成纤维细胞中分离出来，并从生物体包括人或小鼠。这些染色体制剂可进一步用于常规G显带和分子细胞遗传学方法，如荧光原位杂交（FISH），比较基因组杂交（CGH）和光谱核型分析（SKY）。

Tags

Chromosome Preparation Chromosome Analysis Cytogenetic Analysis Chromosome Abnormalities G-banding FISH CGH SKY Cell Culture Metaphase Mitosis Colcemid Hypotonic Solution Carnoy’s Fixative Gene Therapy Stem Cell Research

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