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Tractable Mammalian Cell Infections with Protozoan-primed Bacteria
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Chapters
- 00:05Title
- 02:13Preparation of Legionella pneumophila Cultures for Priming Stage Infections
- 04:14Priming Stage Infection Using Acanthamoeba castellanii
- 07:13Processing of Bacteria for Target Cell Stage Infection after the Priming Stage Infection
- 09:46Experimental Analysis
- 11:14CFU Plating
- 11:51Representative Infection Results
- 13:23Conclusion
This technique provides a method to harvest, normalize and quantify intracellular growth of bacterial pathogens that are pre-cultivated in natural protozoan host cells prior to infections of mammalian cells. This method can be modified to accommodate a wide variety of host cells for the priming stage as well as target cell types.
