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GST-His purification: A Two-step Affinity Purification Protocol Yielding Full-length Purified Proteins
JoVE Journal
Biology
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JoVE Journal Biology
GST-His purification: A Two-step Affinity Purification Protocol Yielding Full-length Purified Proteins
DOI:

11:22 min

October 29, 2013

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Chapters

  • 00:05Title
  • 01:49Recombinant Protein Expression and Preparation of Soluble Cell Lysate
  • 03:17GST Affinity Purification
  • 05:14TALON Metal Affinity Purification
  • 07:00Results: Analysis of GST-His Purification
  • 10:21Conclusion

Summary

Automatic Translation

In the present protocol, we demonstrate a highly efficient and cost-effective small-scale protein purification method, which allows purification of recombinant proteins by uniquely combining a cleavable GST-tag and a small His-tag.

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