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JoVE Journal
Immunology and Infection

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An In vitro Model to Study Heterogeneity of Human Macrophage Differentiation and Polarization
 

An In vitro Model to Study Heterogeneity of Human Macrophage Differentiation and Polarization

Article DOI: 10.3791/50332-v 07:42 min June 12th, 2013
June 12th, 2013

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Summary

Monocyte-derived macrophages are important cells of the innate immune system. Here, we describe an easy to use in vitro model to generate these cells. Using gradient centrifugation, negative bead isolation and specific cell culture conditions, monocyte-derived macrophages can be generated for phenotypic and functional studies.

Tags

Macrophage Differentiation Macrophage Polarization In Vitro Model Peripheral Blood Mononuclear Cells Monocyte Isolation Macrophage Culture Human Macrophages Species-specific Differences
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