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An In vitro Model to Study Heterogeneity of Human Macrophage Differentiation and Polarization
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Immunology and Infection
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JoVE Journal Immunology and Infection
An In vitro Model to Study Heterogeneity of Human Macrophage Differentiation and Polarization

An In vitro Model to Study Heterogeneity of Human Macrophage Differentiation and Polarization

DOI:
10.3791/50332-v

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07:42 min

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June 12, 2013

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, , , , , , ,
1Department of Cardiology,University of Heidelberg

Chapters

  • 00:05Title
  • 01:09PBMC Isolation (Histopaque) and Cell Counting
  • 02:55Negative Isolation of Monocytes
  • 05:09Results: Representative Primary Human Macrophages
  • 07:15Conclusion

Summary

Automatic Translation

Automatic Translation

Monocyte-derived macrophages are important cells of the innate immune system. Here, we describe an easy to use in vitro model to generate these cells. Using gradient centrifugation, negative bead isolation and specific cell culture conditions, monocyte-derived macrophages can be generated for phenotypic and functional studies.

Tags

Macrophage DifferentiationMacrophage PolarizationIn Vitro ModelPeripheral Blood Mononuclear CellsMonocyte IsolationMacrophage CultureHuman MacrophagesSpecies-specific Differences

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